The CaMV 35 S promoter, most commonly used for constitutive expression of foreign proteins in plants is a sequence of 528 base pairs. In the viral genome, the nucleotide sequence of CaMV P6 protein precedes the 35 S promoter sequence. However, the third part of P6 gene sequence overlaps the promoter sequence.
- The 35 S promoter sequence used in transgenic plants does not have ATG at 5’ end nor a translation start site which excludes the possibility of plant cells making the third domain of P6 protein.
- Expression of P6 sequence needs a plant active promoter of its own.
- No scientific literature has been reported on any allergenic properties of CaMV and no similarities have been shown to know allergens.
- Most likely the P6 protein is not an allergen as concluded by Podevin 2012.
- P35S variants do not contain ORFs that encode for proteins that have allergenic or toxic properties.
- These sequences/proteins (like P6) are ubiquitous in nature and have been part of mammalian diets even before human beings evolved.
- We find plant viral genomes in most crops and plant species. This is because plants and viruses have been exchanging genes since millions of years.
- The cauliflower, broccoli, knoll-khol and cabbage we buy in local markets carry CaMV virus due to natural infestation and conatain P6 protein.
- There is a history of safe use of CaMV by humans both in rDNA form and in natural forms.
- Fifty four commercialized GM events (carrying CaMV 35 S Promoter) have passed biosafety tests.
- The possibility of transgenic plants expressing P6 protein (third fragment) can easily be checked by using monoclonal antibodies to P6 protein.
- The quantitative analysis to measure the amount of third fragment of P6 can be carried out.
- Even if the third domain/fragment of P6 is produced in plant cells, it needs to be checked if it is functional.